Journal: Journal of Ovarian Research

Article Title: Exosomes derived from mesenchymal stem cells repair ovarian function by suppressing NLRP3-mediated pyroptosis in cyclophosphamide-induced premature ovarian failure

doi: 10.1186/s13048-025-01785-1

Figure Lengend Snippet: HuMSCs-Exos restored ovarian function and fertility in CTX-induced POF mice. (A) Analysis of the estrous cycle phases showing significant improvements in the proestrus and estrus phases in each group. (B) The number of pregnant mice and (C) the total number of offspring in the POF + Exosomes group, indicating enhanced reproductive capacity following HuMSCs-Exos treatment. (D-G) Hormone level assessments in serum: (D) AMH, (E) E2, (F) FSH, and (G) LH, demonstrating a restoration of hormonal balance in the POF + Exosomes group compared to the POF model group. (H) Western blot analysis of GCs functional proteins (FSHR, AMH, CYP19A1, and FOXL2) in ovarian tissues, with significantly higher expression levels observed in the POF + Exosomes group, indicating the regulatory effects of HuMSCs-Exos on GCs function. * P < 0.05; ** P < 0.01, n = 9

Article Snippet: Membranes were blocked with 5% BSA for 1 h, then incubated overnight at 4 °C with primary antibodies: NLRP3, Caspase-1, IL-1β, IL-18 (1:1000), GAPDH, β-actin (1:5000) from Abcam; CYP19A1 (1/500, Bioss); AMH (1/1000, ABclonal); FSHR (1/1000, Proteintech); Bcl-2 (1/500), Bax (1/1000) from Affinity.

Techniques: Western Blot, Functional Assay, Expressing